Journal: Skin Research and Technology

Article Title: Rutin alleviates psoriasis‐related inflammation in keratinocytes by regulating the JAK2/STAT3 signaling

doi: 10.1111/srt.70011

Figure Lengend Snippet: M5‐induced abnormal differentiation of HaCaT cells was ameliorated following Rutin treatment. (A–C) Western blotting assessed the protein levels of Keratin1 and Keratin5 in M5‐stimulated HaCaT cells and Rutin‐treated M5‐stimulated HaCaT cells. *** p < 0.001 vs. control; ## p < 0.01 and ### p < 0.001 vs. M5. Differential expression testing was performed with ANOVA.

Article Snippet: Subsequently, the membrane underwent blocking, followed by detection with specific primary antibodies, including Keratin5 (Cat#GTX11321, 1:1000, GeneTex, Irvine, CA, USA), Keratin1 (Cat#M01639‐1, 1:5000, BosterBio, Wuhan, China), p‐Janus kinase 2 (JAK2) (Cat#GTX132784, 1:1000, GeneTex), p‐signal transducer and activator of transcription 3 (STAT3) (Cat#bs‐55208R, 1:1000, Bioss, Beijing, China), and β‐actin (Cat#ab8226, 1:10 000, Abcam, Cambridge, Massachusetts, USA).

Techniques: Western Blot, Control, Quantitative Proteomics

Journal: Oncology reports

Article Title: Screening diagnostic biomarkers of OSCC via an LCM-based proteomic approach.

doi: 10.3892/or.2018.6610

Figure Lengend Snippet: Figure 3. Immunohistochemistry analysis of the identified proteins in clinical tissues. (A) Representative images of CK4, CK10/13, CK14 and CK17 immu- nostaining of OSCC tissues accompanied by those of adjacent normal/pre-cancerous lesions. The regions were identified by the pathologist: Adjacent normal regions are shown in blue circles, pre-cancerous lesions are shown in green circles and OSCC regions are shown in red circle. Scale bar, 2,000 µm (left panels), 1,000 µm (middle panels) and 250 µm (right panels). (B) Semi-quantitative scores of CK-positive staining in OSCC tissues and adjacent normal tissues of 20 cases. OSCC, oral squamous cell carcinoma; H&E, hematoxylin and eosin; CK, cytokeratin.

Article Snippet: Antigen retrieval was achieved by boiling the sections in 0.01 M citrate buffer (pH 6.0) in a high-pressure cooker for 3 min. Primary antibodies used in this procedure were as follows: CK4 (rabbit monoclonal; 1:200; cat. no. M07410; Boster Biological Technology, Pleasanton CA, USA); CK14 (rabbit monoclonal; 1:200; cat. no. ZA-0540; ZSGB-BIO, Beijing, China); CK10/13 (mouse monoclonal; 1:30; cat. no. ZM-0314; ZSGB-BIO); CK17 (rabbit monoclonal; 1:200; cat. no. ZA-0551; ZSGB-BIO); and Pan CK (mouse monoclonal; 1:200; cat. no. ZM-0069; ZSGB-BIO).

Techniques: Immunohistochemistry, Staining

Journal: Developmental cell

Article Title: Pulmonary Neuroendocrine Cells Sense Succinate to Stimulate Myoepithelial Cell Contraction

doi: 10.1016/j.devcel.2022.08.010

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit monoclonal anti-KRT20 , Boster Bio , M02828; RRID: AB_2910267.

Techniques: Virus, Recombinant, Plasmid Preparation, Software

Journal: Molecular medicine reports

Article Title: SOX18 knockdown suppresses the proliferation and metastasis, and induces the apoptosis of osteosarcoma cells.

doi: 10.3892/mmr.2015.4541

Figure Lengend Snippet: Figure 1. SOX18 is overexpressed in osteosarcoma tissues, and the knockdown of SOX18 suppresses the proliferation of osteosarcoma cells. (A) mRNA expression levels of SOX18 were significantly increased in the osteosarcoma tissues (n=25), compared with the levels in the normal tissues (n=25), obtained from patients admitted to the Shanghai Tenth People's Hospital (Shanghai, China) between 2009 and 2012. In the graph, a positive log2 Tumor/Normal ratio on the y‑axis indicates increased expression levels of SOX18 in the tumor tissue, whereas a negative log2 Tumor/Normal ratio indicates reduced expression levels of SOX18 in the tumor tissue. (B) Expression levels of SOX18 in five osteosarcoma cell lines were analyzed using western blotting (upper panel) and RT‑qPCR (lower panel). Data are representative of three independent experiments. (C and D) Expression levels of SOX18 in U2OS and MG63 cells was analyzed using western blotting (upper panel) and RT‑qPCR (lower panel). (E and F) Cell proliferation was detected 24, 48 and 72 h subsequent to viral transduction of the U2OS and MG63 cells. Data are representative of three independent experiments and are presented as the mean ± standard deviation (**P<0.01, vs. NC). SOX18, sex‑determining region Y‑box 18; GAPDH, glyceraldehyde 3‑phosphate dehydrogenase; WT, wild‑type; NC, scrambled shRNA; RNAi‑1, SOX18‑shRNA‑1 virus transduction; SOX18‑Ri‑3, SOX18‑shRNA‑3 virus transduction; OD, optical density; RT‑qPCR, reverse transcription‑quantitative polymerase chain reaction.

Article Snippet: The following primary antibodies were used in the present study: Mouse polyclonal SOX18 (Ab66145; 1:1,000), rabbit polyclonal TGF-β1 (Ab92486; 1:400) and rabbit polyclonal RhoA (Ab68826; 1:2,000) (Abcam, Cambridge, MA, USA), rabbit polyclonal PDGF-A (BA0408; 1:200) and rabbit polyclonal PDGF-B (BA0519-2; 1:200) (Wuhan Boster Biological Technology, Ltd. (Wuhan, China) and rabbit monoclonal glyceraldehyde 3-phosphate dehydrogenase (GAPDH; #5174; 1:2,000), Cell Signaling Technology, Inc. (Danvers, MA, USA).

Techniques: Knockdown, Expressing, Western Blot, Transduction, Standard Deviation, shRNA, Virus, Polymerase Chain Reaction

Journal: Molecular medicine reports

Article Title: SOX18 knockdown suppresses the proliferation and metastasis, and induces the apoptosis of osteosarcoma cells.

doi: 10.3892/mmr.2015.4541

Figure Lengend Snippet: Figure 2. SOX18 RNAi induces S‑phase arrest and apoptosis in osteosarcoma cells. The U2OS and MG63 cells were transduced with the indicated virus and were collected after 48 h. (A and B) Cell cycle profile was analyzed using flow cytometry. (C and D) Cells were stained with annexin V‑FITC/PI, and apoptotic rates was analyzed using flow cytometry. Data are representative of a minimum of three independent experiments and are presented as the mean ± standard deviation (**P<0.01, vs. NC). SOX18, sex‑determining region Y box 18; WT, wild‑type; NC, scrambled shRNA transduction; SOX18‑Ri‑3, SOX18‑shRNA‑3 virus transduction; PI, propidium iodide; FITC, fluorescein isothiocyanate.

Article Snippet: The following primary antibodies were used in the present study: Mouse polyclonal SOX18 (Ab66145; 1:1,000), rabbit polyclonal TGF-β1 (Ab92486; 1:400) and rabbit polyclonal RhoA (Ab68826; 1:2,000) (Abcam, Cambridge, MA, USA), rabbit polyclonal PDGF-A (BA0408; 1:200) and rabbit polyclonal PDGF-B (BA0519-2; 1:200) (Wuhan Boster Biological Technology, Ltd. (Wuhan, China) and rabbit monoclonal glyceraldehyde 3-phosphate dehydrogenase (GAPDH; #5174; 1:2,000), Cell Signaling Technology, Inc. (Danvers, MA, USA).

Techniques: Transduction, Virus, Cytometry, Staining, Standard Deviation, shRNA

Journal: Molecular medicine reports

Article Title: SOX18 knockdown suppresses the proliferation and metastasis, and induces the apoptosis of osteosarcoma cells.

doi: 10.3892/mmr.2015.4541

Figure Lengend Snippet: Figure 4. Expression levels of TGF‑β1, PDGF‑A, PDGF‑B and RhoA are downregulated by SOX18 RNAi. The protein and mRNA levels of the indicated genes were evaluated using (A and B) western blotting and (C and D) reverse transcription‑quantitative polymerase chain reaction in the U2OS and MG63 cells. Data are presented as the mean ± standard deviation (**P<0.01, vs. NC). TGF‑β1, transforming growth factor‑β1; PDGF‑A, platelet‑derived growth factor‑A; RhoA, Ras homolog family member A; GAPDH, glyceraldehyde 3‑phosphate dehydrogenase; SOX18, sex‑determining region Y‑box 18; WT, wild‑type; NC, scrambled shRNA virus transduction; SOX18‑Ri‑3, SOX18‑shRNA‑3 virus transduction.

Article Snippet: The following primary antibodies were used in the present study: Mouse polyclonal SOX18 (Ab66145; 1:1,000), rabbit polyclonal TGF-β1 (Ab92486; 1:400) and rabbit polyclonal RhoA (Ab68826; 1:2,000) (Abcam, Cambridge, MA, USA), rabbit polyclonal PDGF-A (BA0408; 1:200) and rabbit polyclonal PDGF-B (BA0519-2; 1:200) (Wuhan Boster Biological Technology, Ltd. (Wuhan, China) and rabbit monoclonal glyceraldehyde 3-phosphate dehydrogenase (GAPDH; #5174; 1:2,000), Cell Signaling Technology, Inc. (Danvers, MA, USA).

Techniques: Expressing, Western Blot, Polymerase Chain Reaction, Standard Deviation, shRNA, Virus, Transduction

Journal: Molecular medicine reports

Article Title: SOX18 knockdown suppresses the proliferation and metastasis, and induces the apoptosis of osteosarcoma cells.

doi: 10.3892/mmr.2015.4541

Figure Lengend Snippet: Figure 3. Silencing of SOX18 inhibits the metastasis of osteosarcoma cells and reduces tumor growth in vivo. The U2OS and MG63 cells were transduced with the indicated virus. Adhesion ability was analyzed using a cell adhesion assay. (A) Representative images and (B) quantitative results of the cell adhe sion assay. The U2OS and MG63 cells were transduced with the indicated virus and cell invasion was analyzed in Matrigel‑coated Transwell chambers. (C) Representative images and (D) quantitative results of the cell adhesion assay. Data are representative of a minimum of three independent experiments and are presented as the mean ± standard deviation (**P<0.01, vs. NC); magnification, x100. SOX18, sex‑determining region Y‑box 18; WT, wild‑type; NC, scrambled shRNA virus transduction; SOX18‑Ri‑3, SOX18‑shRNA‑3 virus transduction.

Article Snippet: The following primary antibodies were used in the present study: Mouse polyclonal SOX18 (Ab66145; 1:1,000), rabbit polyclonal TGF-β1 (Ab92486; 1:400) and rabbit polyclonal RhoA (Ab68826; 1:2,000) (Abcam, Cambridge, MA, USA), rabbit polyclonal PDGF-A (BA0408; 1:200) and rabbit polyclonal PDGF-B (BA0519-2; 1:200) (Wuhan Boster Biological Technology, Ltd. (Wuhan, China) and rabbit monoclonal glyceraldehyde 3-phosphate dehydrogenase (GAPDH; #5174; 1:2,000), Cell Signaling Technology, Inc. (Danvers, MA, USA).

Techniques: In Vivo, Transduction, Virus, Cell Adhesion Assay, Standard Deviation, shRNA